reverse transcription-quantitative polymerase chain reaction(English)
- the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data, as it allows the detection of rare transcripts and the observation of small variations in gene expression. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo biopsies. However, this approach does not correct differences in RNA integrity
- mRNA, RNA
- Clinical chemistry
- https://doi.org…1471-2199-10-31