puromycin-associated nascent chain proteomics(English)

• used to characterize new protein synthesis in C2C12 myotubes and changes resulting from their exposure to the inflammatory mediators lipopolysaccharide (LPS) and interferon (IFNγ). Important, as inflammation in muscle induces the synthesis of mediators that can impair protein synthesis and enhance proteolysis, and when sustained lead to muscle atrophy
• IFNγ, LPS
• Myocyte / muscle, Metabolism, Inflammasome
• https://doi.org…753-022-01622-3