galactosylsphingosine(English)

• to achieve separation of GlcSph and GalSph it is necessary to switch to a HILIC stationary phase for the chromatography so that interactions are dominated by the polar sugar moiety. GlcSph was found to elute before GalSph. In the control samples there was ∼3-fold more GlcSph than GalSph. In NP-C patient samples, the increase above normal levels was dominated by GlcSph, leading to an increase in the GlcSph/GalSph ratio (to >12); aka 3H-galactosyl sphingosine
• GALC, GlcSph, SPC, Gb3, HILIC, NP-C
• Biochemistry
• https://doi.org…al.pone.0114669